RNA Switch™ series


RNA Switch™ series is product series utilizing “RNA Switch technology” which was invented by Prof. Hirohide Saito who works at CiRA, Kyoto University.
RNA Switch can regulate expression of genes in a cell by detecting microRNA (miRNA) which exists in a cell and have various roles for cellular mechanisms. One of the major features is that cells can distinguish the active state of miRNAs in living cells and it can be used for regenerative medicine by using an RNA switch for cell-type specific active miRNAs identified by this tool. It enables identification and selection of differentiated target cells, such as cardiomyocytes, and application as a searching tool for disease-related miRNAs.

RNA Switch™ is an artificially synthesized mRNA that contains an array that recognizes miRNAs and a marker gene. This series enables identification and selection of cells by using marker genes such as fluorescent proteins and suicide genes. It is possible to purify only specific cells without using a cell sorter or other devices and can be easily introduced into cells by the same process as normal transfection.

Product list


Product name Cat.No. Size Storage SDS 
Control detector RNA Switch™ P-0001 9μg -80℃ Download
CM detector RNA Switch™ P-0002 9μg -80℃ Download
iPSC detector RNA Switch™ P-0003 9μg -80℃ Download
CM purifier RNA Switch™ P-0004 9μg -80℃ Download
iPSC purifier RNA Switch™(in preparation) P-0005 9μg -80℃
iPSC eliminator RNA Switch™ P-0006 9μg -80℃ Download
puro resistant mRNA™ P-0007 9μg -80℃ Download

※The amount guarantees minimum 9μg.

CM detector RNA Switch™(P-0002)&CM purifier RNA Switch™(P-0004)


CM detector RNA Switch™ can be used for cardiomyocytes.

CM detector RNA Switch™ uses a fluorescent protein as a marker gene that can be used as a fluorescence confirmation tool and can be used with the Control Detector RNA Switch™ (P-0001) as a control.

Application data

Protocol

CM purifier RNA Switch™ can be used for cardiomyocytes.

CM purifier RNA Switch™ is designed for a cell selection tool because it uses an apoptosis protein as a marker gene and should be co-introduced with puro- resistant mRNA ™ (P-0007) to eliminate non-transfected cells. This makes it possible to select cells with high efficiency.

Application data

Protocol

iPSC detector RNA Switch™(P-0003)


iPSC detector RNA Switch™ is a product that can be used as a fluorescence confirmation tool because it uses a fluorescent protein as a marker gene and can be used with Control Detector RNA Switch™ (P-0001) as a control.

Application data

Protocol

iPSC eliminator RNA Switch™(P-0006)


iPSC eliminator RNA Switch™ uses puromycin resistance protein as a marker gene and can only remove iPS cells by using the puromycin.

Before             After

Selection result by iPSC eliminator RNA Switch™(The part shown by”i“in the above figure is iPS cell)

Application data

Protocol


References
Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches.
Cell Stem Cell. 2015 May 20. pii: S1934-5909(15)00163-0. doi: 10.1016/j.stem.2015.04.005. PMID: 26004781
Miki K, Endo K, Takahashi S, Funakoshi S, Takei I, Katayama S, Toyoda T, Kotaka M, Takaki T, Umeda M, Okubo C, Nishikawa M, Oishi A, Narita M, Miyashita I, Asano K, Hayashi K, Osafune K, Yamanaka S, Saito H, Yoshida Y.

MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells.
Scientific Reports. 2016 Sep 9;6:32532. doi: 10.1038/srep32532. PMID: 27608814
Parr CJ, Katayama S, Miki K, Kuang Y, Yoshida Y, Morizane A, Takahashi J, Yamanaka S, Saito H.