RNA Switch™ series
RNA Switch™ series is product series utilizing “RNA Switch technology” which was invented by Prof. Hirohide Saito who works at CiRA, Kyoto University.
RNA Switch can regulate expression of genes in a cell by detecting microRNA (miRNA) which exists in a cell and have various roles for cellular mechanisms. One of the major features is that cells can distinguish the active state of miRNAs in living cells and it can be used for regenerative medicine by using an RNA switch for cell-type specific active miRNAs identified by this tool. It enables identification and selection of differentiated target cells, such as cardiomyocytes, and application as a searching tool for disease-related miRNAs.
RNA Switch™ is an artificially synthesized mRNA that contains an array that recognizes miRNAs and a marker gene. This series enables identification and selection of cells by using marker genes such as fluorescent proteins and suicide genes. It is possible to purify only specific cells without using a cell sorter or other devices and can be easily introduced into cells by the same process as normal transfection.
|Control detector RNA Switch™||P-0001||9μg||-80℃||Download|
|CM detector RNA Switch™||P-0002||9μg||-80℃||Download|
|iPSC detector RNA Switch™||P-0003||9μg||-80℃||Download|
|CM purifier RNA Switch™||P-0004||9μg||-80℃||Download|
|iPSC purifier RNA Switch™（in preparation）||P-0005||9μg||-80℃||–|
|iPSC eliminator RNA Switch™||P-0006||9μg||-80℃||Download|
|puro resistant mRNA™||P-0007||9μg||-80℃||Download|
※The amount guarantees minimum 9μg.
CM detector RNA Switch™(P-0002)＆CM purifier RNA Switch™(P-0004)
CM detector RNA Switch™ can be used for cardiomyocytes.
CM detector RNA Switch™ uses a fluorescent protein as a marker gene that can be used as a fluorescence confirmation tool and can be used with the Control Detector RNA Switch™ (P-0001) as a control.
CM purifier RNA Switch™ can be used for cardiomyocytes.
CM purifier RNA Switch™ is designed for a cell selection tool because it uses an apoptosis protein as a marker gene and should be co-introduced with puro- resistant mRNA ™ (P-0007) to eliminate non-transfected cells. This makes it possible to select cells with high efficiency.
iPSC detector RNA Switch™(P-0003)
iPSC detector RNA Switch™ is a product that can be used as a fluorescence confirmation tool because it uses a fluorescent protein as a marker gene and can be used with Control Detector RNA Switch™ (P-0001) as a control.
iPSC eliminator RNA Switch™(P-0006)
iPSC eliminator RNA Switch™ uses puromycin resistance protein as a marker gene and can only remove iPS cells by using the puromycin.
Selection result by iPSC eliminator RNA Switch™（The part shown by”i“in the above figure is iPS cell）
Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches.
Cell Stem Cell. 2015 May 20. pii: S1934-5909(15)00163-0. doi: 10.1016/j.stem.2015.04.005. PMID: 26004781
Miki K, Endo K, Takahashi S, Funakoshi S, Takei I, Katayama S, Toyoda T, Kotaka M, Takaki T, Umeda M, Okubo C, Nishikawa M, Oishi A, Narita M, Miyashita I, Asano K, Hayashi K, Osafune K, Yamanaka S, Saito H, Yoshida Y.
MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells.
Scientific Reports. 2016 Sep 9;6:32532. doi: 10.1038/srep32532. PMID: 27608814
Parr CJ, Katayama S, Miki K, Kuang Y, Yoshida Y, Morizane A, Takahashi J, Yamanaka S, Saito H.